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. 2014 May 22;12(5):3072–3090. doi: 10.3390/md12053072

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© 2014 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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Apoptotic induction induced by 10AB involves mitochondrial dysfunction. The effect of 10AB treatment on the mitochondrial membrane potential (MMP) in HL 60 cells was evaluated. (A,B) Cells were treated with 10AB (1.5, 3.0, and 6.0 μM) for 24 h. Quantitative results showed a significant increase in HL 60 cells with disturbed MMP in response to the use of 10AB. Results are presented as means ± SD of three independent experiments (** p < 0.01; *** p < 0.001). The effect of 10AB on the expression of apoptotic; (C) and mitochondrial metabolism; (D) related proteins in HL 60 cells. The bands were quantified via densitometry and normalized to β-actin levels; (E) Merged images of Mitotracker with cytochrome c immunofluorescence suggested that the increase in cytochrome c was the direct effect of 10AB treatment at 1.5 of 3 μM; (F) Cell lysates were subjected to immunoprecipitation with anti-Bax6A7 antibody and analyzed by immunoblotting with anti-Bax antibody.