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. 2014 May 22;12(5):3072–3090. doi: 10.3390/md12053072

Figure 4.

Figure 4

The apoptotic induction of 10AB in HL 60 cells involves ROS production. We evaluated the effect of the 10AB treatment on ROS generation in HL 60 cells. (A) Cells were treated with 10AB (6.0 μM) for the indicated times. Quantitative results showed a gradual increase in the ROS production in response to the 10AB treatment when compared with the control group. We also evaluated the effect of ROS generation on the 10AB-induced apoptosis in HL 60 cells. Cells were pretreated with 3.0 mM NAC for 2 h, then treated with 3.0 or 6.0 μM of 10AB; The apoptotic populations (B) and the disruption of MMP (C) were examined with annexin-V/PI and JC-1 staining assay. Results are presented as mean ± SD of three independent experiments (** p < 0.001).