Figure 5.

Increased accumulation of fibrocytes and CCR5-positive cells in fibrotic mouse dermis is reversed by CSD. Mice were treated with bleomycin and CSD as described in Figure 3. Skin sections were stained using the indicated antibodies and DAPI to detect nuclei. Large numbers of double-positive cells were observed in bleomycin-treated mice (that did not receive CSD) for each comparison (A, CCR5 vs. Procol I; B, Procol I vs. CD45; C, CCR5 vs. CD45), while almost none were observed in Control mice. CSD treatment almost completely blocked the accumulation of these cells. These representative fields were photographed at 400 × magnification. To quantify these observations, the number of double-positive cells was counted in three subjects per category, five HPF per subject. The data are presented in terms of the number of double-positive cells per HPF (average ± s.e.m.). (D) quantification of (A); (E) quantification of (B); (F) quantification of (C).