Table 1.
Characteristics of different setups combining HOTs with a confocal module
| Microscope | Tam et al. (17) |
Oddos et al. (13) |
Yevnin et al. (18) |
This work |
|---|---|---|---|---|
| Inverted 1 objective | Inverted 1 objective | Upright/inverted 2 objectives | Inverted 1 objective | |
| Imaging module | ||||
| Confocal head | Spinning | Scanning | Spinning | Spinning |
| Frame rate | ∼30 ms | ∼300 ms | ∼30 ms | ∼30 ms |
| Trapping module | ||||
| Directionality | No | 2D (xy) | 3D (xyz) | 3D (xyz) |
| Number of trap | 1 | 2 | Up to 10 | Up to 10 |
| Independent traps | – | 1 | Multiple | Multiple |
| Independent from the imaging module | No | No | Yes | Yes |