Figure 4. Tcf3 induction rescues the defective migration in Stat3-deficient cells.

(a) Primary keratinocytes from Stat3^fl/fl (cont) or Stat3^fl/fl;K14-Cre (Stat3 cKO) mice were isolated and were transduced with GFP-tagged lentiviral vector expressing tet-inducible Tcf3 or with empty vector control. After the transduced cells were enriched by fluorescent cell sorting, they were grown to confluence and then subjected to a migration assay with or without Dox. Images of keratinocytes 48hrs after initiation of the migration assay. Black bar denotes 200μm.

(b) Graph quantifying the relative area the cells migrated normalized over vehicle control. For each sample, over 30 non-overlapping fields were measured at each timepoint; and each experiment was repeated three times. Data are the mean ± s.d. **p<0.001 (Student’s t-test).