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. Author manuscript; available in PMC: 2014 Dec 9.
Published in final edited form as: Nat Commun. 2014 Jun 9;5:3965. doi: 10.1038/ncomms4965

Fig 2. Genetic interaction of SET2 with representative DNA damage response and repair genes.

Fig 2

Representative DNA damage response and repair genes were selected from the Open Biosystems gene knockout collection and were verified by either phenotypic analysis or PCRs (using primers specific for the gene deletion) or both. Subsequent double mutants were made by gene replacement. (A) Qualitative representation of genetic interactions (on MMS) of SET2 with DNA damage response and repair genes. Genes have been clustered together, manually, based on their annotated function in the Saccharomyces Genome Database (SGD). Black lines represent no genetic interaction, red lines indicate synthetic sick, and green lines indicate same pathway interaction. The entire screen was repeated twice. (B) Serial dilutions of representative strains show that set2Δ is sick with rad51Δ and asf1Δ while the set2Δ is epistatic to both dot1Δ and yku70Δ on phleomycin. Individual spotting assays were done in triplicate plates (at once) and the set of triplicates were repeated twice.