Figure 3. Multiple I470V mutations are required to modify the energetics of N-type inactivation.

Top: cartoons of selected characterized mutants, where cyan colour represents a subunit mutated to I470V. Bottom: representative current traces from mutants cartooned above. All currents are in response to a voltage step to +60 mV from a holding potential of −80 mV, and overlaid with best-fits to a Markov kinetic model. Horizontal bar: 10 ms; Vertical bars (from left to right): 400, 100, 100 and 100 pA.