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. Author manuscript; available in PMC: 2014 Dec 4.
Published in final edited form as: Nat Commun. 2014 Jun 4;5:3998. doi: 10.1038/ncomms4998

Figure 4. Snail1 Regulates Angiogenic Program in a Xenograft Transplant Model.

Figure 4

(a) Representative image of isolated ECs from eGFP+;Snail1fl/fl mice. Scale bar: 50 μm.

(b) Adeno-β-gal or Adeno-Cre infected ECs were stimulated with vehicle or VEGF (100 ng/ml) for 12 h and subjected to Western blot analysis. Results are representative of 3 or more experiments.

(c,d) Whole-mount PECAM-1 (CD 31) staining of the implants after 14 d in vivo. Scale bar: 50 μm.

(c,g,I,k) Gross view of implants. Scale bar: 2 mm.

(f,h,i,l) Whole-mount PECAM-1 staining of the implants. Scale bar: 100 μm.

(m) Quantification of relative vessel length in the implants (n = 3 in each group). Data are presented as mean ± SEM. **, $$, ##p < 0.01, ANOVA test.