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. Author manuscript; available in PMC: 2015 Jul 1.
Published in final edited form as: Cell Calcium. 2014 Apr 27;56(1):25–33. doi: 10.1016/j.ceca.2014.04.003

Figure 1. Expression of hSERT in cultured skeletal muscle cells.

Figure 1

Myoblasts isolated from wild type (Wt) or dysgenic (MDG) mice were transduced with the hSERT encoding retrovirus. The transduced cells were selected as described in Materials and methods. The cells were differentiated into myotubes and the hSERT expression was evaluated by immunofluorescence visualized by confocal microscopy (scale bar = 20 μm). The hSERT signal is shown in green and the DAPI nuclear staining is shown in blue for Wt-transduced (A), Wt-control (B), MDG-transduced (C), and MDG-control myotubes (D). E, Western blot that shows the expression of hSERT in samples from myotubes transduced with the hSERT-retrovirus (+) or uninfected cells (−). (F and G) The hSERT activity was evaluated measuring the uptake of APP+ by fluorescence microscopy. F, Wt transduced myotubes (black trace) and the effect of fluoxetine (gray trace, FLX). Non-transduced Wt myotubes (red trace) and the effect of fluoxetine (FLX, blue trace) are plotted. G is the same as F but performed in dysgenic (MDG) myotubes. The traces shown are mean ± s.e.m. of n ≥ 25 cells for each condition.