Figure 3. PB composition after BMT.

Representative flow cytometric analyses (five-percentile contour plots) of PB lymphocytes (gated on forward- and side-scatter) from normal BN (far left) and PVG.7B rats (far right) are juxtaposed with the composition of host (RT7.B^–) and donor (RT7.B⁺)-derived cells from BN rats 14 days after allogeneic T cell-depleted BMT and prior to DLI (PVG.7B>BN; cf Fig. 1). T cell (CD3⁺NKR-P1^–), NK cell (CD3^–NKR-P1⁺), and NKT cell (CD3⁺NKR-P1⁺) populations (middle row) were gated. Only few T cells derived from PVG.7B donor BM cells were present in the PB of transplanted rats, whereas host T cells and donor NK cells (bold squares) were predominant. T cells were further divided into CD4 (CD4⁺CD8^–), CD8 (CD4^–CD8⁺), as well as “double-positive” (CD4⁺CD8⁺) subpopulations (bottom row). Host-derived T cells were mostly CD4⁺CD8^–. Numbers indicate the relative frequencies (% of parent) of the respective gates.