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. Author manuscript; available in PMC: 2015 Feb 13.
Published in final edited form as: Cell Rep. 2014 Jan 23;6(3):499–513. doi: 10.1016/j.celrep.2013.12.039

Figure 5. Runx1 initiates a hair germ early progenitor fate in bulge cells.

Figure 5

(A, A′) QRT-PCR of sorted bulge cells show robust down-regulation of several bulge stem cell markers tested. (B) Skin sections from K15-EGFP;Runx1iTG mice at PD20 show telogen morphology and GFP expression in the HG and in some bulge (Bu) cells. (C) FACS dot plot show sorting gates to isolate bulge (as CD34+/α6+/GFP+/- cells) and HG cells as (CD34−/α6+/GFP+). The cut-off for HG GFP was for the brightest 1/3 of the cells to avoid potential contamination from dimmer cells occasionally found in epidermis and sebaceous gland (not shown). (D) Microarray analysis of wild type (WT) and iTG bulge (Bu) and hair germ (HG) sorted populations; shown are number and probes which decrease or increase among each paired comparison. (E) Comparison of bulge iTG/WT by microarrays shows changes in bulge (Bu), hair germ (HG) and matrix gene signature. Note down-regulation of bulge signature and up-regulation of HG signature in iTG bulge. The effect on matrix signature was neutral. See also Fig. S5C. (F, G) GO categories enriched among Runx1 targets in two selected gene subsets, as indicated.