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. 2014 May 19;2014:341529. doi: 10.1155/2014/341529

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Copyright © 2014 Ai-Ling Cui et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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(a) Effect of humanin on NMDA triggered dynamic changes of cytoplasmic Ca²⁺ concentration. The fluorescence intensity which is related to cytoplasmic Ca²⁺ concentration was captured and recorded every 660 ms for 300 successive measurements (for about 198 s) for each subject. 5 to 10 neurons were measured in every group. The curves are formed by the connection of 300 data which stand for the average instantaneous fluorescence intensity of each group. HN only: the addition of 10 μM HN itself in the medium. HN (0.1 μmol/L) and HN (1 μmol/L) groups: HN (0.1 μmol/L) or HN (1 μmol/L) in NMDA treatment groups. (b) Effect of humanin on NMDA triggered dynamic changes of cytoplasmic Ca²⁺ concentration. Picture 1 to picture 9 stand for the typical successive cellular morphological characteristics of HN (1 μmol/L) on NMDA-triggered increase of cytoplasmic Ca²⁺ concentration.