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Morphological changes and N : C ratio analysis of 8305C cells following 0.1 and 1 mmol/L VPA treatment for 96 h. (a) Untreated control cells (DMSO) showed spindle and mesenchymal morphology. (b) and (c) 0.1 and 1 mmol/L VPA treated cells showed oval-round to polygonal morphology (magnification 40x). (d) High N : C ratio of the untreated cells decreased dramatically in VPA treated cells. Data are shown as mean ± SD. ***P < 0.001.
