Table 1.
Primer sequences for quantitative RT-PCR.
| Gene | Accession number | Forward primer (5′-3′) | Reverse primer (5′-3′) | Size (bp) |
|---|---|---|---|---|
| HPRT | NM_000194 | TGGACAGGACTGAACGTCTTG | CCAGCAGGTCAGCAAAGAATTTA | 111 |
| NIS | NM_000453 | TGCGGGACTTTGCAGTACATT | TGCAGATAATTCCGGTGGACA | 133 |
| TTF-1 | NM_001079668 | AGCACACGACTCCGTTCTC | GCCCACTTTCTTGTAGCTTTCC | 68 |
| p21 | NM_000389 | CCTGTCACTGTCTTGTACCCT | GCGTTTGGAGTGGTAGAAATCT | 130 |
| OCT-4 | NM_001173531 | CTTGAATCCCGAATGGAAAGGG | GTGTATATCCCAGGGTGATCCTC | 164 |
| hTERT | NM_001193376 | AACCTTCCTCAGCTATGCCC | GCGTGAAACCTGTACGCCT | 210 |
| PAX-8 | NM_003466 | TGGGGACTACAAACGCCAGA | GCTGTCCATAGGGAGGTTGAAT | 163 |
| c-MYC | NM_002467 | CCACAGCAAACCTCCTCACAG | GCAGGATAGTCCTTCCGAGTG | 105 |
| MMP-2 | NM_004530 | CTTCCAAGTCTGGAGCGATGT | TACCGTCAAAGGGGTATCCAT | 119 |
| MMP-9 | NM_004994 | GGGACGCAGACATCGTCATC | TCGTCATCGTCGAAATGGGC | 139 |
HPRT: Hypoxanthine phosphoribosyltransferase 1; NIS: sodium/iodide symporter; TTF1: thyroid transcription factor 1; p21: CIP1/WAF1; OCT-4: octamer-binding transcription factor 4; hTERT: human telomerase reverse transcriptase; PAX8: Paired box gene 8; c-MYC: v-myc myelocytomatosis viral oncogene homolog; MMP-2: matrix metallopeptidase 2; MMP-9: matrix metallopeptidase 9.