Table 1.
Combined coa gene typing and HaeIII RFLP patterns of the 75 isolated MRSA strains.
| coa band types (3) | coa gene subtypes (10) | RFLP pattern (12) | Size of PCR product (approximate bp) | Size of HaeIII fragments (approximate bp) | Total isolates number (%) | Isolate serial number |
|---|---|---|---|---|---|---|
|
Co1 (1 band)
16/75 (21.3%) |
Co1a | C1 (3 bands) | 810 | 2 (162), 486 | 3 (4) | 7, 12, 13 |
| Co1b | A1 (uncut) | 891 | 891 | 2 (2.7) | 43, 49 | |
| B (2 bands) | 891 | 324, 567 | 3 (4) | 56, 62, 68 | ||
| C2 (3 bands) | 891 | 2 (162), 567 | 7 (9) | 51–55, 57, 58 | ||
| Co1c | A2 (uncut) | 1215 | 1215 | 1 (1.3) | 46 | |
|
| ||||||
|
Co2 (2 bands)
54/75 (72%) |
Co2a | D1 (4 bands) | 405, 810 | 2 (162), 405, 486 | 44 (58.7) | 1–6, 9–11, 16, 18–20, 22–28, 30–35, 37–42, 45, 47, 50, 60, 61, 65, 66, 71–75 |
| Co2b | D2 (4 bands) | 405, 729 | 81, 162, 405, 486 | 1 (1.3) | 17 | |
| Co2c | D3 (4 bands) | 405, 891 | 2 (162), 405, 567 | 7 (9) | 44, 48, 59, 63, 67, 69, 70 | |
| Co2d | C3 (3 bands) | 81, 810 | 81, 2 (405) | 1 (1.3) | 14 | |
| Co2e | E1 (5 bands) | 405, 972 | 81, 162, 324, 405, 486 | 1 (1.3) | 36 | |
|
| ||||||
|
Co3 (3 bands)
5/75 (6.7%) |
Co3a | E2 (5 bands) | 81, 405, 810 | 81, 2 (162), 405, 486 | 4 (5.3) | 8, 15, 21, 29 |
| Co3b | A3 (uncut) | 243, 486, 1215 | 243, 486, 1215 | 1 (1.3) | 64 | |