Table 2.
Combined spa gene typing and HaeII RFLP patterns of the 75 isolated MRSA strains.
| spa band types (4) | spa gene subtypes (11) | RFLP pattern By HaeII (12) | Size of PCR product (approximate bp) | Size of HaeII fragments (approximate bp) | Total isolates number (%) | Isolate serial number |
|---|---|---|---|---|---|---|
|
S1 (1 band)
63/75 (84%) |
S1a | A1 (uncut) | 192 | 192 | 1 (1.3) | 43 |
| S1b | B1 | 1104 | 2 (240), 600 | 3 (4) | 44, 69, 70 | |
| S1c | B2 | 1200 | 2 (240), 696 | 21 (28) | 1–3, 15, 17, 45, 47, 48, 50, 57–59, 63, 66, 67, 71–75 | |
| B3 | 1200 | 2 (192), 792 | 1 (1.3) | 55 | ||
| S1d | B4 | 1392 | 2 (240), 912 | 37 (49.3) | 4–13, 18–35, 37, 38, 40–42, 51–54 | |
|
| ||||||
|
S2 (2 bands)
7/75 (9.3%) |
S2a | A2 (uncut) | 144, 600 | 144, 600 | 1 (1.3) | 64 |
| S2b | C1 | 408, 1296 | 2 (192), 312, 1008 | 1 (1.3) | 14 | |
| S2c | B5 | 240, 1200 | 2 (240), 912 | 3 (4) | 60, 61, 65 | |
| S2d | C2 | 240, 1392 | 2 (240), 504, 792 | 1 (1.3) | 62 | |
| S2e | C3 | 360, 1392 | 100, 240, 504, 912 | 1 (1.3) | 68 | |
|
| ||||||
|
S3 (3 bands)
1/75 (1.3%) |
S3 | D | 360, 1104, 1392 | 150, 2 (240), 792, 456, 912 | 1 (1.3) | 36 |
|
| ||||||
|
S4 (no band)
4/75 (5.3%) |
S4
No band |
E | — | — | 4 (5.3) | 39, 46, 49, 56 |