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. 2014 May 18;2014:786234. doi: 10.1155/2014/786234

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Copyright © 2014 Peng Cui et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Adipogenic differentiation of DMS/PCs. (a) DMS/PCs morphology and staining detection of the induced group and the control group. ((B), (D), and (F)) The control group of adipogenic differentiation. After 1 d, 7 d, and 14 d, the cells cultured in complete medium had no difference in the morphology and phenotype and were also negative for oil red O staining. (A) DMS/PCs induced in the inducing culture medium after 1 d, and there is no difference in the morphology and phenotype. (C) The induced group of adipogenic differentiation. After induction for 7 days, the cell morphology began to change from a shuttle shape to an oblate shape, and many intracellular lipid droplets were present in the induced cells. (E) The cells of induced group were positive for oil red O staining (bar = 25 μm). (b) RT-PCR detection of the adipogenic markers LPL and PPAR-γ expression in induced group and control group. Induced cells were positive for LPL and PPAR-γ, but the control cells were not. 1: GAPDH; 2: LPL; 3: PPAR-γ.