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. 2014 May 18;2014:786234. doi: 10.1155/2014/786234

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Copyright © 2014 Peng Cui et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Neurogenic differentiation of DMS/PCs. After 10 days of induction, neural-like cells with a multipolar spindle-like shape were observed. Neural-specific markers including β-III tubulin (red), NF (red), nestin (green), and MAP-2 (green) were detected through immunofluorescence. (a) DMS/PCs morphology and identification of endothelial cells by immunofluorescent labeling. ((a1), (a2), (a3), and (a4)) Phase contrast. ((b1), (b2), (b3), and (b4)) β-III tubulin+, NF+, nestin+, and MAP-2+. ((c1), (c2), (c3), and (c4)) Merge (bar = 25 μm). (b) The cells in the control group did not express the neurogenic-specific makers. ((d1), (d2), (d3), and (d4)) Phase contrast. ((e1), (e2), (e3), and (e4)) β-III tubulin-, NF−, nestin−, and MAP-2−. ((f1), (f2), (f3), and (f4)) Merge (bar = 25 μm).