Figure 2. Various proteins induce similar shear stress responses.

Cells were seeded on slides coated with collagen type-IV (COL), fibronectin (FN), or laminin (LM), cultured under static conditions for two days, and then for two days either exposed to 5.0 dyne/cm² of shear stress (SHEAR samples) or maintained in culture under static conditions (STATIC controls). Phase images of cells for both STATIC controls and SHEAR samples on COL, FN, and LM are shown. flk1, tie2, and runx1 gene expression was assessed for SHEAR samples (■) and normalized to trial-matched STATIC controls (□). Data presented are mean ± SEM (n=7-12), where significant differences between STATIC and SHEAR groups are indicated with asterisks (** p<0.01 and *** p<0.001). Scale bar represents 100Lm.