Figure 7. Delayed exposure to shear stress is less effective at promoting a hematopoietic phenotype.
Cells were cultured under static conditions for 2 or 3 days before four days of either STATIC (striped white bars) or SHEAR (τ=5.0 dyne/cm2, striped black bars) treatment. Fluorescence of both STATIC and SHEAR samples were assessed and compared to flow cytometry control samples to calculate the percentage of cells positive for markers of differentiation. Assessed proteins were FLK1 (A), TIE2 (B), RUNX1 (C), and CD41 (D). Data presented are mean ± SEM (n=3-4), where significant differences between STATIC and SHEAR groups were determined by a t-test and indicated by asterisks (* p<0.05 and *** p<0.001).