Figure 8. FLK1 inhibition negates the effects of shear stress on early differentiation.
Cells were exposed to two days of STATIC (□) or SHEAR (τ=5.0 dyne/cm2, ■) treatment, where media from select STATIC and SHEAR samples was supplemented with a FLK1 inhibitor (SU14-8: 4.5Lg/ml) during treatment. Gene expression was assessed and each value was normalized to the average value of the trial-matched STATIC controls. Genes assessed include tie2 (A) and runx1 (B). Data presented are mean ± SEM (n=3-4), where significant differences between trial-matched STATIC and SHEAR are indicated by asterisks (*** p<0.001).