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. 2014 May 29;4:47. doi: 10.1186/s13568-014-0047-6

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Copyright © 2014 Lohse et al.; licensee Springer

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited.

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Penetration assay withB. bassianaATP-02. Spray formulations with (n = 7) and without (n = 2) B. bassiana (Bb) were brushed onto the leaf tips. (a) Microscopic detection of Bb in a cross-section of a leaf mid rip from a Bb-treated leaf at 200-fold magnification. The arrow marks the hyphae in the leaf tissue. (b)Bb was detected in the untreated area of the Bb-treated leaves by PCR and subsequent gel electrophoresis. Lane 1–5: DNA from Bb-treated leaves; Lane 6: Bb DNA from pure SDA culture; Lane 7–8: DNA from control-treated leaves; Lane 9: no template control; Lane 10: 100 bp DNA ladder (AppliChem GmbH, Darmstadt, Germany).