Table 2.
Oligonucleotide primers
| Primer name | Sequence (5′- 3′) | Restriction enzyme |
|---|---|---|
| pknG-up-In-F |
GCCAAGCTTgcatgcATGAAGGATAATGAAGATTTCGATCCAGATTCACCAGC |
SphI |
| pknG-up-In-R-801 |
ACCATTTGTGTCGCCGGCTTTGCAGCGGTCTTTCAGGGA |
|
| pknG-down-In-F-1669 |
GCCAAGCTTgcatgcGGCGACACAAATGGTTCTCCG |
SphI |
| pknG-down-In-R |
AAAAGGATCggatccCTAGAACCAACTCAGTGGCCGCA |
BamHI |
| pknG-In-F2 |
CCAGTGCCAAGCTTgcatgcATGAAGGATAATGAAGATTTCGATCCAGATTCACCAGC |
SphI |
| pknG-In-R2 |
AAAAAGGATCggatccCTAGAACCAACTCAGTGGCCGCA |
BamHI |
| SacI-gadB-F |
GGCgagctcATGTTTAAAGCTGTTCTGTTGGGCAA |
SacI |
| XhoI-gadBF-R | CCGctcgagTTACTTGTCATCGTCATCCTTGTAGTCAGGTCGGAACTACTCGATTCACG | XhoI |
Restriction enzyme cleavage sites are shown in small letters, and complementary sequences of the primer pairs used for overlap-extension PCR are shown in italics.