| ChIP sample, or 5-50 ng of WCE | 30.0 μl |
| Water | 45.0 ul |
| T4 DNA ligase buffer(NEB, B0202S) | 10.0 μl |
| dNTP mix,each 10 mM (NEB, N0447L) | 4.0 μl |
| T4 DNA polymerase (NEB, M0203L) | 5.0 μl |
| Klenow DNA polymerase (NEB, M0210L) | 1.0 μl |
| T4 PNK (NEB, M0201L) | 5.0 μl |
| Total | 100.0 μl |
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