| DNA sample | 23.0 μl |
| Phusion Master Mix with HF Buffer (NEB, F-531L) | 25.0 ul |
| Genomic PCR primer 1.1 (Illumina) | 1.0 μl |
| Genomic PCR primer 2.1 (Illumina) | 1.0 μl |
|
| |
| Total | 50.0 μl |
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