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. Author manuscript; available in PMC: 2014 Jun 11.
Published in final edited form as: Methods. 2009 Mar 9;48(3):240–248. doi: 10.1016/j.ymeth.2009.03.001
Stock 1x Mix
10X Thermopol buffer (NEB) 5.0 μl
dNTP mix (25 mM each) 0.5 μl
Primer 1.2 for reampi (10 μM) 2.5 μl
Primer 2.2 for reampii (10 μM) 2.5 μl
AmpliTaq 1.0 μl
ddH2O 36.5 μl
Total 48.0 μl