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. 2014 Jun 11;4:5260. doi: 10.1038/srep05260

Figure 5. PHF6 knockdown in combination with TMZ treatment decreases cell viability.

Figure 5

(a), PHF6 mRNA expression in U87-R1 and Hs683-R1 cells 24 hrs after transfection with siPHF6 or siNT as determined by qRT-PCR. (b), representative images of the different treatment conditions (CTRL, TMZ, siNT/CTRL, siNT/TMZ, siPHF6/CTRL, siPHF6/TMZ) in Hs683-R1, U87-R1, and LNZ308-R1 cells four days after treatment with TMZ. The panels show DAPI-stained nuclei. Size bar, 20 µm. (c), quantification of cell viability of Hs683-R1, U87-R1, and LNZ308-R1 cells after treatment with siPHF6 or siNT in the presence or absence of 50 µM (Hs683-R1), 300 µM (U87-R1) or 400 µM (LNZ308-R1) TMZ. Shown are averages, error bars indicate SD. *p<0.05 student's t test. * in c indicates statistical significance of combination of PHF6 knockdown and TMZ compared to PHF6 knockdown or TMZ treatment alone.