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. 2014 Jun 1;28(11):1159–1164. doi: 10.1101/gad.237479.114

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© 2014 Park et al.; Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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Figure 2. — Functional Ig genes preferentially colocalize in transcription factories. (A,B) Four-color 3D DNA immuno-FISH images of selected 0.3-μm-thick optical sections (Z-slices) of the same plasma cell nuclei (outlined by white dashed lines) from either wild-type (WT) (A) or homozygous Vκ8-Jκ5 knock-in mice (B). (Blue) IgH (VhDh)i; (green) IgH; (red) IgJ or Igκ; (white) α-Pol II. (C) Cartoon summarizing the quantification of DJ and VDJ rearranged IgH alleles that were localized within <1.0 μm from both Pol II and either IgJ or Igκ alleles. Data are from 300 and 306 plasma cells analyzed from wild-type and homozygous Vκ8-Jκ5 knock-in mice, respectively, in three independent experiments (see also Supplemental Table 5 for primary data and P-values from χ² statistical tests).