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. 2014 Jun 1;28(11):1159–1164. doi: 10.1101/gad.237479.114

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© 2014 Park et al.; Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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Figure 4. — Transcripts originating from different Ig genes exhibit features of nuclear organization favorable for export to the cytoplasm. (A–C) Representative four-color 3D RNA immuno-FISH images of the same 0.3-μm optical sections of individual plasma cells with peripherally localized transcribing Ig genes. Primary transcripts are indicted by green, red, or yellow arrows. (A) (Blue) DAPI; (white) α-Lam B; (green) Igκi; (red) Cκ. (B) (Blue) DAPI; (white) α-Lam B; (red) IgHi; (green) Cμ. (C) (Blue) DAPI; (white) α-Lam B; (yellow) IgJi; (red) C_J. (D,E) (Red) Cκ; (green) Igκi; (blue) Cμ or C_J; (yellow) IgHi or IgJi; (white) DAPI. White arrows depict interchromatin channels occupied by processed Ig gene transcripts. Primary transcripts of colocalized alleles are indicated by green or yellow arrows. (D) Representative five-color 3D RNA FISH images of the same 0.3-μm optical section of a plasma cell, with interiorly colocalized Ig genes and the nucleus outlined by white dashed lines. (E) Representative reconstructed five-color 3D RNA FISH image of a plasma cell with interiorly colocalized Ig genes.