Figure 2.

In vitro transcription termination at the Rho-dependent pgaA terminator. (A) Schematic representation of the DNA template used for the in vitro transcriptions. Key transcript features are also depicted above the template regions. (B,C) Representative gels show the formation of truncated transcripts resulting from Rho-dependent termination at the pgaA site under “low-salt” (50 mM KCl) transcription conditions (B) or after activation by the CsrA and NusG proteins under “high-salt” (150 mM KCl) conditions (C).