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. 2014 May 26;14:171. doi: 10.1186/1472-6882-14-171

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Copyright © 2014 Park et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver ( http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Time-of-addition effect on HRV-4 replication in HeLa cells. Approximately twofold quantities of the IC₅₀ values of ellagic acid (EA) and ribavirin (RN) were applied. The 50 μg/mL EA and 100 μg/mL RN were added at various times preinfection (-1 h), coinfection (0 h), or postinfection (1, 2, 4, and 6 h) of human rhinovirus-4 (HRV-4) to HeLa cells at 37°C. After 2 days, inhibition was evaluated by SRB method and expressed as the inhibition rate. Each bar represents the mean ± SD of triplicate samples of three independent experiments.