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. 2014 May 12;7:69. doi: 10.1186/1754-6834-7-69

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Copyright © 2014 de Jaeger et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Starch screening and content determination. (A) Iodine vapour staining on starchless mutants. C. reinhardtii strain BAFJ5 was included as a control. The staining was performed after 9 days of nitrogen deprivation (TAP-N plates) to enhance the visibility of the iodine staining which is normally interfered by the chlorophyll. Starch content (% of DW) for (B) nitrogen-replete and (C) nitrogen-depleted conditions over time. The values are derived from biological duplicate cultures. Deviation from the duplicate average is indicated by the error bars. DW, dry weight; slm, starchless mutant; TAP, Tris-Acetate-Phosphate; wt, wild type.