Table 1.
GC/MS data for sterol profiling from N. oceanica treated with sterol biosynthetic inhibitors
| Sterols | Structure | RT (min) | RRTc |
Sterol profile (%) |
|||||
|---|---|---|---|---|---|---|---|---|---|
| Control | CLO | TBF | TEB | TDM | 25-AZA | ||||
| Squalene |
1 |
10.25 |
0.755 |
-* |
- |
12.0 |
- |
- |
- |
| Cholesterol |
2 |
13.58 |
1.000 |
74.0 |
70.4 |
61.6 |
62.0 |
56.2 |
81.3 |
| Cholest-8-enol |
3 |
13.75 |
1.012 |
- |
- |
- |
- |
8.2 |
- |
| Desmosterol |
4 |
13.91 |
1.024 |
- |
- |
- |
- |
- |
3.0 |
| Pollinastanol |
5 |
14.33 |
1.056 |
- |
- |
- |
- |
13.1 |
- |
| 4, 14-Dimethylcholest-8-enol |
6 |
14.53 |
1.070 |
- |
- |
- |
1.7 |
- |
- |
| 24-Methylenecholesterol |
7 |
14.59 |
1.074 |
1.4 |
2.5 |
0.7 |
2.7 |
- |
4.0 |
| 24(25)-Dihydrolanosterol |
8 |
15.18 |
1.117 |
- |
- |
- |
1.7 |
- |
- |
| 4-Methylpollinasterol |
9 |
15.47 |
1.139 |
- |
- |
- |
- |
3.7 |
- |
| 24-Methylcholesta-5, 25(27)-dienol |
10 |
15.51 |
1.142 |
0.9 |
1.0 |
0.9 |
0.8 |
0.3 |
1.0 |
| Fucosterol |
11 |
15.68 |
1.155 |
10.1 |
10.1 |
10.3 |
7.2 |
6.8 |
6.0 |
| Obtusifoliol |
12 |
15.73 |
1.158 |
- |
- |
- |
0.2 |
- |
- |
| Isofucosterol |
13 |
15.91 |
1.171 |
13.5 |
16.1 |
14.3 |
8.9 |
9.3 |
4.8 |
| Stigmasta-8, 24(28) E-dienol |
14 |
15.93 |
1.173 |
- |
- |
- |
- |
0.4 |
- |
| Cycloartenol |
15 |
16.06 |
1.182 |
- |
- |
- |
12.8 |
- |
- |
| Stigmasta-8, 24(28) Z-dienol |
16 |
16.16 |
1.190 |
- |
- |
- |
- |
1.9 |
- |
| Cycloartanol | 17 | 16.59 | 1.222 | - | - | - | 2.1 | - | - |
Cultures in exponential phase were diluted in fresh media and grown for 12 h under 50 μmol photons m-2 s-1 light. Subsequently, cultures were treated with inhibitors at a concentration of IC50 or an equivalent amount of DMSO (not exceeding a final concentration of 0.1%). Cell aliquots were collected for sterol profiling four days later. Three biological replicates of algal cultures were established under each treatment. Abbreviations: RRTc, relative retention time to cholesterol; CLO, clomazone; TBF, terbinafine; TEB, tebuconazole; TDM, tridemorph; 25-AZA, 25-azalanosterol. The asterisk (*) indicates a value below the detection limit under current experimental conditions. The structure numbers in boldface are listed in Additional file 2: Figure S3.