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. 2014 Jun 3;13:91. doi: 10.1186/1476-511X-13-91

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Copyright © 2014 Takechi et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Semi-quantitative 3-D immunomicroscopy for cerebrovascular and neuronal inflammation. Cerebrovascular and neuronal inflammation was assessed by 3-D immuno quantitative analysis of the cerebral parenchymal and vascular expressions of GFAP and COX-2 in cortex (CTX) and hippocampus (HPF) of mice fed with low-fat (LF) or saturated fat (SFA) diet with/without probucol for 3 and 12 months. (A) Mean voxel intensity of parenchymal GFAP and COX-2 in CTX and HPF is shown. * indicates the statistical significance assessed with one-way ANOVA followed by Tukey’s post hoc test (p < 0.05, n = 8). (B) Pearson’s correlation coefficient between the GFAP or COX-2 expression and cerebral IgG/apoB extravasation in each animal was also performed (n = 24).