Skip to main content
. 2014 May 13;2014:421486. doi: 10.1155/2014/421486

Figure 2.

Figure 2

Pentamer formation of CTB-Fim2. (a) SDS-PAGE with purified CTB-Fim2. Lane 1 is the sample boiled in reducing conditions while lane 2 is the same protein sample not subjected to boiling and in nonreducing conditions. Bands of 36 and 180 kDa of CTB-Fim2 are shown, respectively. (b) GM1-ELISA to verify the ability of the pentamer to bind GM1-ganglioside using recombinant CTB-Fim2. Commercial CTB was used as a positive control. The 96-well plates were coated with GM1 or BSA and mouse anti-CTB antisera (1 : 2500).