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Specific IgG antibodies in serum. Induction of serum anti-Fim2 IgG after intraperitoneal (a) or intranasal (b) immunization with PBS, CTB, rFim2, and CTB-Fim2. Anti-Fim2 IgG titers of individual serum collected immediately before challenge on day 36 are shown. Values represent the OD at 492 nm and show the mean (±standard error) of 8 animals (**P < 0.001 versus rFim2 IN; ***P < 0.0001 versus PBS and CTB IN and IP).
