Figure 1.

Comparison between SMYB1 and other SMYB isoforms. The coding sequences for all SMYB proteins were aligned to two segments of the S. mansoni genome scaffold 000610, in order to identify exonic and intronic portions of the genes. Genes are represented by gray bars, while exons are represented by colored boxes and introns by connecting lines. Numbers depicted on the gray bars are relative to nucleotide positions and numbers below the exons are relative to amino acid positions on the resulting protein sequence. (A) For SMYBX (Smp_097750), there are three exons and the third (C-terminal, blue box) one is separated from the first two (dark green and yellow boxes) by a very long intron. (B) Regarding its paralogs SMYB1 (Smp_097800.1) is formed by four exons and the first two (green and orange boxes) are shared with isoforms SMYB2 (Smp_097800.2, with one extra nucleotide at the end of the second exon) and SMYB3 (Smp_097800.3). While the SMYB2 isoform contains an exon (light blue box) similar to SMYB1 exon 4 (navy blue box), but longer (and in a different reading frame), SMYB3 contains a third exon (light red) which is similar to SMYB1 exon 3 (dark red), but longer. (C) Muscle alignment of SMYB1 (Q27277), SMYB2 (G4LXD2), SMYB3 (G4LXD0), and the SMYBX_putative (G4LXC4). Disordered regions are highlighted in red, according to Disopred predictions and the conserved domain is underlined in blue.