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. 2014 Jun 11;5:174. doi: 10.3389/fgene.2014.00174

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Copyright © 2014 Dias, Boroni, Rocha, Dias, de Laet Souza, Oliveira, Bitar, Macedo, Machado, Caliari and Franco.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Kinetics of specific anti-rSMYB1 IgG, IgG1, and IgG2a antibodies in sera from mice vaccinated with rSMYB1. Sera were collected from 10 immunized mice per group prior to the first immunization and at days 10, 25, and 40 after the first immunization and assayed by ELISA. The results are presented as the mean absorbance at 492 nm for each isotype on each day of sera collection. Asterisks indicate statistically significant differences between the vaccinated and preimmune groups: ^*p < 0.05; ^**p < 0.01. Black bars: IgG antibody. Gray bars: IgG1 antibody. White bars: IgG2a antibody. The results are representative of two independent biological replicates.