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. 2014 Jun 11;5:174. doi: 10.3389/fgene.2014.00174

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Copyright © 2014 Dias, Boroni, Rocha, Dias, de Laet Souza, Oliveira, Bitar, Macedo, Machado, Caliari and Franco.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Kinetics of specific anti-rSMYB1, anti-SWAP and anti-SEA IgG, IgG1, and IgG2a antibodies. The presence of antibodies was measured in sera from 10 mice immunized with Tris-NaCl + adjuvant (placebo group) or with rSMYB1 + adjuvant (immune group). Sera were collected prior to sacrifice 45 days after challenge and assayed by ELISA. The results are presented as the mean absorbance at 492 nm for each immunoglobulin isotype. Asterisks indicate statistically significant differences between the vaccinated and pre-immune groups: ^*p < 0.05, ^**p < 0.01. DAC, days after challenge. Black bars: IgG antibody. Gray bars: IgG1 antibody. White bars: IgG2a antibody. The results are representative of two independent biological experiments.