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Quantification of the effective field-of-view accessed through the glass plug via (a) the 4X 0.28 NA objective, and (b) the 40X 0.6 NA objective. The distance from the center of the plug to the boundary at which fluorescence falls to half its maximum value is approximately 540 µm for the 4X objective and 490 µm for the 40X objective. These correspond to fields-of-view that are 70% and 60% of the plug diameter, respectively.
