Table 1.
Effect of punicalagin on the 2-aminofluorene induced mutagenicity in Salmonella typhimurium.
| Treatment | Dose (μM) | Number of His+ revertants colonies/plate (mean ± SE) | |||
|---|---|---|---|---|---|
| TA 97a | TA 98 | TA 100 | TA 102 | ||
| Spontaneous | 144.0 ± 8.7 | 39.7 ± 3.8 | 140.3 ± 10.8 | 325.7 ± 16.6 | |
| Positive control (2AF) | 1.5 μg | 336.7 ± 14.4 | 254.0 ± 11.8 | 510.3 ± 23.9 | 1475.0 ± 38.1 |
| aPunicalagin | 50 | 132.3 ± 8.8 | 40.7 ± 2.9 | 136.0 ± 11.7 | 320.0 ± 21.9 |
| 100 | 158.0 ± 7.4 | 36.3 ± 1.8 | 162.7 ± 15.0 | 341.0 ± 18.3 | |
| 250 | 165.7 ± 10.9 | 31.0 ± 1.7 | 175.3 ± 14.3 | 356.0 ± 10.4 | |
| 500 | 188.0 ± 9.5 | 22.3 ± 1.8 | 191.0 ± 15.0 | 358.0 ± 19.9 | |
| bPunicalagin + 2AF | 50 | 304.7 ± 19.8 (15.7) | 210.3 ± 10.7 (20.5) | 426.7 ± 28.3 (22.4) | 1028.0 ± 21.8*** (38.7) |
| 100 | 270.3 ± 10.1* (37.1) | 172.3 ± 12.0** (37.5) | 358.0 ± 12.2** (43.8) | 854.0 ± 31.6*** (54.8) | |
| 250 | 246.7 ± 12.3** (52.6) | 122.0 ± 9.9** (59.2) | 304.3 ± 19.5** (61.5) | 660.0 ± 24.7*** (72.8) | |
| 500 | 220.0 ± 9.3*** (78.5) | 65.3 ± 4.1*** (81.4) | 245.0 ± 9.6*** (83.1) | 563.0 ± 29.4*** (81.6) | |
|
| |||||
| R 2 | 0.98 | 0.99 | 0.99 | 0.99 | |
aNegative control; bpreincubation test; values in parenthesis are % inhibition of mutagenicity.
*P < 0.05; **P < 0.005 and ***P < 0.001; 2AF: 2-aminofluorene; R 2: linear regression analysis.