Table 5.
Effect of ellagic acid on the 2-aminofluorene induced mutagenicity in Salmonella typhimurium.
| Treatment | Dose (μM) | Number of His+ revertants colonies/plate (mean ± SE) | |||
|---|---|---|---|---|---|
| TA 97a | TA 98 | TA 100 | TA 102 | ||
| Spontaneous | 144.0 ± 8.7 | 39.7 ± 3.8 | 140.3 ± 10.8 | 325.7 ± 16.6 | |
| Positive control (2AF) | 1.5 μg | 336.7 ± 14.4 | 254.0 ± 11.8 | 510.3 ± 23.9 | 1475.0 ± 38.1 |
| aEllagic acid | 50 | 142.3 ± 10.7 | 50.0 ± 5.1 | 136.3 ± 12.0 | 336.0 ± 23.0 |
| 100 | 159.7 ± 10.5 | 44.7 ± 3.8 | 162.7 ± 16.6 | 275.0 ± 16.8 | |
| 250 | 175.0 ± 15.0 | 38.3 ± 2.3 | 178.0 ± 12.7 | 308.0 ± 17.2 | |
| 500 | 184.0 ± 10.6 | 32.0 ± 3.6 | 188.7 ± 12.8 | 302.0 ± 17.1 | |
| bEllagic acid + 2AF | 50 | 312.3 ± 20.5 (12.5) | 214.3 ± 13.9 (19.4) | 426.0 ± 18.6* (22.5) | 1276.0 ± 38.0* (17.5) |
| 100 | 296.7 ± 13.4 (22.6) | 171.0 ± 9.5** (39.6) | 350.0 ± 12.8** (46.1) | 924.0 ± 25.3*** (45.9) | |
| 250 | 264.0 ± 14.2* (44.9) | 132.7 ± 9.3** (56.3) | 285.3 ± 13.1** (67.7) | 610.0 ± 26.2*** (74.1) | |
| 500 | 212.0 ± 10.4** (81.7) | 76.3 ± 6.1*** (80.0) | 240.7 ± 9.8*** (83.8) | 431.0 ± 13.6*** (89.0) | |
|
| |||||
| R 2 | 0.93 | 0.99 | 0.99 | 0.98 | |
aNegative control; bpreincubation test; values in parenthesis are % inhibition of mutagenicity.
*P < 0.05; **P < 0.005 and ***P < 0.001; 2AF: 2-aminofluorene; R 2: linear regression analysis.