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Example of an intact circulating tumor cell (CTC) (A), a granular CTC (B) and of images with no CTCs (C,D,E,F) as they display the same morphology in all channels (C,D) or have a nucleus larger than the cytoplasm or not 50% inside the cytoplasm and cytokeratin staining not surrounding the nucleus (E,F). DAPI, 4',6-diamidino-2-phenylindole.
