Table 2. 2H NMR Quadrupolar Splittings (Δνq, in kHz) for Labeled Alanine CD3 Groups in F5GWALP23, F4,5GWALP23, and Y4GWALP23a.
| DLPC |
DMPC |
DOPC |
||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Ala-d4 | F5 | F4,5 | Y4 | W5W18 | F5 | Y4 | F5 | F4,5 | Y4 | W5W18 |
| 7 | 17.8 | 23.7 | 15.8 | -- | -- | 17.6 | 16.2 | 16.2 | 11.8 | -- |
| 9 | 20.4 | 23.5 | 9.2 | 14.9 | -- | 6.4 | 2.8 | 0.8 | 1.5 | 6.6 |
| 11 | 21.9 | 25.7 | 18.8 | -- | -- | 17.9 | 17.6 | 18.6 | 16.4 | 15.4 |
| 13 | 13.8 | 19.6 | 9.2 | 9.0 | -- | 8.5 | 3.4 | 0.8 | 3.2 | 2.3 |
| 15 | 18.3 | 23.4 | 18.8 | 25.6 | -- | 18.4 | 17.6 | 18.6 | 18.4 | 5.6 |
| 17 | 1.0 | 1.8 | 1.3 | 21.6 | -- | 2.7 | 1.0 | 1.9 | 2.2 | 17.2 |
a
Sample orientation is β = 0°. Each value (in kHz) is the average of the magnitude observed at β = 0° and twice the magnitude observed for a β = 90° sample orientation. Values that are absent (--) were not recorded. The labeled alanines are identified, and the positions of the N-flanking aromatic amino acids are indicated as F5, F4,5, and Y4. The C-flanking W19 is present in all samples except for W5W18.