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Production of NO from BAECs treated with NAC, BH4, or both after H/R. (A and B) Relative production of NO from eNOS. Treating cells with 100 μM BH4 together with 2 mM NAC resulted in >85% NO preservation as compared to the control. NAC treatment alone also significantly preserved NO but to a lesser extent. *H/R vs control, H/R+NAC, H/R+NAC+BH4: p < 0.002. #H/R+NAC vs H/R+NAC+BH4: p < 0.003.
