Table.

Details of the Primary and Secondary Antibodies Used in Immunofluorescence Studies

Primary Antibody and Catalog Number	Vendor	Concentration	Use as Marker	Secondary Antibody
G. simplicifolia isolectin-B4 (B-1105)	Vector Labs, Burlingame, CA, USA	1:133	Marker for blood vessels	Texas Red avidin; Vector Labs
Collagen IV, rabbit polyclonal (SAB4500369)	Sigma-Aldrich, St. Louis, MO, USA	1:200	Marker for blood vessels	Alexafluor 555 (donkey anti-rabbit); Invitrogen, Eugene, OR, USA
CD105 (endoglin) rat anti-mouse (Lot 75619)	BD Biosciences, San Jose, CA, USA	1:250	Marker for angiogenesis	Alexafluor 555 (donkey anti-rat); Invitrogen
VEGF mouse monoclonal IgM (AB 38909)	Abcam, Cambridge, MA, USA	1:250	Marker for angiogenesis	Alexafluor 488 (donkey anti-mouse); Invitrogen
GFAP rabbit polyclonal (Lot# 00019620)	DakoCytomation, Carpenteria, CA, USA	1:100	Marker for stressed  Müller cells and  astrocytes	Alexafluor 488 (goat anti-rabbit); Invitrogen
CD11b/c equivalent antibody mouse monoclonal (Ab 33827)	Abcam	1:200	Marker for microglia	Alexafluor 555 (donkey anti-mouse); Invitrogen
Vimentin goat anti-human (AB1620)	Millipore, Billerica, MA, USA	1:250	Marker for Müller cells	Alexafluor 555 (mouse anti-goat); Invitrogen
Mouse monoclonal(AB 18401)	Abcam	1:200	Isotype control  (VEGF study)	Alexafluor 488 (donkey anti-mouse); Invitrogen