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. 2012 Dec 5;14(6):R153. doi: 10.1186/bcr3366

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Copyright © 2012 Cañas et al.; licensee BioMed Central Ltd.

This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Subcellular localization of ERα is altered by the impairment of S-nitrosothiol homeostasis. (A) To determine subcellular localization of ERα and p53, estrogen-deprived MCF-7 cells were exposed for 6 hours to the indicated treatments and stained for ERα (green) and p53 (red) immunofluorescence and counterstained with 4',6-diamidino-2-phenylindole (DAPI; blue). Merged images of ERα, p53 and DAPI staining are also shown. Original magnification: 40 ×. Aur, auranofin; CSNO, S-nitrosocysteine; ER, estrogen receptor.