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. 2012 Dec 6;14(6):R154. doi: 10.1186/bcr3367

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Copyright © 2012 Rezaei et al.; licensee BioMed Central Ltd.

This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Neural (N)-cadherin silencing in Ep5ExTu cells induced epithelial (E)-cadherin expression and influenced its localization in the cells in a dose-dependent manner. (a) Phase contrast microscopy of control cell lines (Sh-Scr) and Sh-N-cadherin cell lines (Sh-N-cad1.1, Sh-N-cad1.2 and Sh-N-cad2). Bar, 100 μm. (b) E-cadherin mRNA levels in Ep5ExTu clones. (c) The graph represents the quantification of the relative amount of E-cadherin mRNA in three independent experiments. β-actin was used as loading control. The mean ± SD is indicated. (n = 3) (**, P < 0.0012 and ***, P < 0.0009). (d) Immunolocalization of E-cadherin in Ep5 (Bar, 30 μm) and Ep5ExTu (Bar, 60 μm) clones by immunofluorescence microscopy. Nuclear staining with DAPI is also shown.