Skip to main content
. Author manuscript; available in PMC: 2014 Jun 11.
Published in final edited form as: J Immunol. 2009 Aug 26;183(6):3690–3699. doi: 10.4049/jimmunol.0900953

Figure 8.

Figure 8

XBP-1-deficient plasmablasts synthesize significantly less phosphatidylcholine (PC), sphingomyelin (SM), and phosphatidylinositol (PI). Naïve B cells purified from the spleens of either XBP-1WT or XBP-1KO mice were cultured with LPS for 4 days. Lipids were extracted from at least 100 million cells. Quantitation of each lipid was carried out using liquid chromatography/mass spectrometry. Lipid abundance was calculated by comparing to internal standards. Experiments were repeated for four times using B cells pooled from 9 mice of each genotype. Data are presented as mean±SD. Lipids analyzed include phosphatidylcholine (PC), ceramide (Cer), sphingomyelin (SM), phosphatidylethanolamide (PE), phosphatidylinositol (PI), phosphatidylserine (PS) and phosphatidylglycerol (PG).