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Mitochondrial staining using rhodamine 123 of human lung cancer cells A549 in the presence or absence of the methanolic fraction of S. grandiflora leaves. (a) Untreated A549 cells. (b) A549 cells treated with IC50 dose of the methanolic fraction for 24 h showing decreased membrane potential as indicated by the arrows. (c) DAPI costaining of A549 cells treated with IC50 dose of the methanolic fraction. Magnification 200x.
